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pucmini icap php b plasmid addgene cat  (Addgene inc)


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    Structured Review

    Addgene inc pucmini icap php b plasmid addgene cat
    Pucmini Icap Php B Plasmid Addgene Cat, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/addgene+cat/pUCmini-iCAP-PHP%2EB+(Plasmid+%23103002)/pm41916278-261-283-285
    Average 94 stars, based on 18 article reviews
    pucmini icap php b plasmid addgene cat - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Transfection:

    Article Title: Adopting orphan receptors: zebrafish Tlr4 homologs mediate responses to group IX/X transition metals
    Article Snippet: Human embryonic kidney (HEK) 293T cells (ATCC, catalog (cat) number CRL-3216) were grown in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with FBS (10%) and penicillin-streptomycin (100 μg/mL) at 37°C and 5% CO 2 . .. 24 hours after seeding, the HEK293T cells were transfected with an empty vector (pcDNA3-2xHA) or co-transfected with zebrafish Tlr4ba, Tlr4bb (a generous gift from Dr. Victoriano Mulero, Spain) , or a human TLR4 expression clone (Addgene, cat. #13018). .. 24 hours after seeding, the HEK293T cells were transfected with an empty vector (pcDNA3-2xHA) or co-transfected with zebrafish Tlr4ba, Tlr4bb (a generous gift from Dr. Victoriano Mulero, Spain) , or a human TLR4 expression clone (Addgene, cat. #13018).

    Article Title: Evaluation and experimental monitoring of calcium in plasma membrane inner nanoscale cytoplasm regions.
    Article Snippet: .. For some experiments, before staining, HEK cells were transiently transfected with an expression vector encoding NCX1 (Rat NCX1.1 (pcDNA3.1+), Addgene Cat. #75190) or with GCaMP6s (pGP-CMV-GCaMP6s-CAAX, Addgene Cat. #52228) using GenJect-40 reagent (dia-m.ru, Russia) according to standard manufacturer protocol. ..

    Article Title: Evaluation and experimental monitoring of calcium in plasma membrane inner nanoscale cytoplasm regions
    Article Snippet: .. For some experiments, before staining, HEK cells were transiently transfected with an expression vector encoding NCX1 (Rat NCX1.1 (pcDNA3.1+), Addgene Cat. #75190) or with GCaMP6s (pGP-CMV-GCaMP6s-CAAX, Addgene Cat. #52228) using GenJect-40 reagent (dia-m.ru, Russia) according to standard manufacturer protocol. ..

    Expressing:

    Article Title: Adopting orphan receptors: zebrafish Tlr4 homologs mediate responses to group IX/X transition metals
    Article Snippet: Human embryonic kidney (HEK) 293T cells (ATCC, catalog (cat) number CRL-3216) were grown in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with FBS (10%) and penicillin-streptomycin (100 μg/mL) at 37°C and 5% CO 2 . .. 24 hours after seeding, the HEK293T cells were transfected with an empty vector (pcDNA3-2xHA) or co-transfected with zebrafish Tlr4ba, Tlr4bb (a generous gift from Dr. Victoriano Mulero, Spain) , or a human TLR4 expression clone (Addgene, cat. #13018). .. 24 hours after seeding, the HEK293T cells were transfected with an empty vector (pcDNA3-2xHA) or co-transfected with zebrafish Tlr4ba, Tlr4bb (a generous gift from Dr. Victoriano Mulero, Spain) , or a human TLR4 expression clone (Addgene, cat. #13018).

    Article Title: Evaluation and experimental monitoring of calcium in plasma membrane inner nanoscale cytoplasm regions.
    Article Snippet: .. For some experiments, before staining, HEK cells were transiently transfected with an expression vector encoding NCX1 (Rat NCX1.1 (pcDNA3.1+), Addgene Cat. #75190) or with GCaMP6s (pGP-CMV-GCaMP6s-CAAX, Addgene Cat. #52228) using GenJect-40 reagent (dia-m.ru, Russia) according to standard manufacturer protocol. ..

    Article Title: Lentiviral-based vectors and related systems and methods for eukaryotic gene editing
    Article Snippet: 18 pSaCas93xms2 Plasmid expressing A synthetic dsDNA oligo was generated by annealing SaCas9 mRNA with oligo 3xloop-F (SEQ ID NO: 45) and oligo 3xloop-R three MS2 stem loops at (SEQ ID NO: 46), which was then inserted by Infusion TM the 3′ UTR reaction (Clontech)into the EcoRI site of pSaCas9- 2xMS2. .. 19 pSaCas912xms2 Plasmid expressing The EcoR1-Xho1 fragment from pSL-MS2-12X SaCas9 mRNA with (Addgene Cat. No. 27119), encoding the 12 MS2 stem twelve MS2 stem loops loops, was inserted between the EcoR1-SalI sites of at the 3′ UTR pSaCas9-1xMS2, which is after the stop codon of SaCas9 but before the polyA signal sequence. ..

    Article Title: Evaluation and experimental monitoring of calcium in plasma membrane inner nanoscale cytoplasm regions
    Article Snippet: .. For some experiments, before staining, HEK cells were transiently transfected with an expression vector encoding NCX1 (Rat NCX1.1 (pcDNA3.1+), Addgene Cat. #75190) or with GCaMP6s (pGP-CMV-GCaMP6s-CAAX, Addgene Cat. #52228) using GenJect-40 reagent (dia-m.ru, Russia) according to standard manufacturer protocol. ..

    shRNA:

    Article Title: Optimizing PDIA1 Inhibition as a Strategy to Inhibit NLRP3 Inflammasome Activation and Activity
    Article Snippet: The following plasmids were used for viral transfection: REV (pRSV-rev; Addgene cat. 12253), RRE (pMDLRRE; Addgene cat. 12251), VSV-G (pMD2. .. G; Addgene cat. 12259), and Mission shRNA plasmid targeting PDIA1 in pLKO.1 vector (Sigma cat. TRCN0000049194). .. The plasmid of shRNA scrambled (nonsilencing) in pLKO.1 vector was a gift from Jacob Corn (pLKO.1-puro-shNT; Addgene plasmid cat. 109012) 34 .

    Plasmid Preparation:

    Article Title: Optimizing PDIA1 Inhibition as a Strategy to Inhibit NLRP3 Inflammasome Activation and Activity
    Article Snippet: The following plasmids were used for viral transfection: REV (pRSV-rev; Addgene cat. 12253), RRE (pMDLRRE; Addgene cat. 12251), VSV-G (pMD2. .. G; Addgene cat. 12259), and Mission shRNA plasmid targeting PDIA1 in pLKO.1 vector (Sigma cat. TRCN0000049194). .. The plasmid of shRNA scrambled (nonsilencing) in pLKO.1 vector was a gift from Jacob Corn (pLKO.1-puro-shNT; Addgene plasmid cat. 109012) 34 .

    Article Title: Evaluation and experimental monitoring of calcium in plasma membrane inner nanoscale cytoplasm regions.
    Article Snippet: .. For some experiments, before staining, HEK cells were transiently transfected with an expression vector encoding NCX1 (Rat NCX1.1 (pcDNA3.1+), Addgene Cat. #75190) or with GCaMP6s (pGP-CMV-GCaMP6s-CAAX, Addgene Cat. #52228) using GenJect-40 reagent (dia-m.ru, Russia) according to standard manufacturer protocol. ..

    Article Title: Lentiviral-based vectors and related systems and methods for eukaryotic gene editing
    Article Snippet: 18 pSaCas93xms2 Plasmid expressing A synthetic dsDNA oligo was generated by annealing SaCas9 mRNA with oligo 3xloop-F (SEQ ID NO: 45) and oligo 3xloop-R three MS2 stem loops at (SEQ ID NO: 46), which was then inserted by Infusion TM the 3′ UTR reaction (Clontech)into the EcoRI site of pSaCas9- 2xMS2. .. 19 pSaCas912xms2 Plasmid expressing The EcoR1-Xho1 fragment from pSL-MS2-12X SaCas9 mRNA with (Addgene Cat. No. 27119), encoding the 12 MS2 stem twelve MS2 stem loops loops, was inserted between the EcoR1-SalI sites of at the 3′ UTR pSaCas9-1xMS2, which is after the stop codon of SaCas9 but before the polyA signal sequence. ..

    Article Title: Evaluation and experimental monitoring of calcium in plasma membrane inner nanoscale cytoplasm regions
    Article Snippet: .. For some experiments, before staining, HEK cells were transiently transfected with an expression vector encoding NCX1 (Rat NCX1.1 (pcDNA3.1+), Addgene Cat. #75190) or with GCaMP6s (pGP-CMV-GCaMP6s-CAAX, Addgene Cat. #52228) using GenJect-40 reagent (dia-m.ru, Russia) according to standard manufacturer protocol. ..

    Virus:

    Article Title: Bioengineering mini-colons for ex vivo colorectal cancer research.
    Article Snippet: Tumor initiation remains one of the least understood events in cancer biology, largely due to the challenge of dissecting the intricacy of the tumorigenic process in laboratory settings.. The insufficient biological complexity of conventional in vitro systems makes animal models the primary experimental approach to study tumorigenesis.. Despite providing valuable insights, these in vivo models function as experimental black boxes with limited spatiotemporal resolution of cellular dynamics during oncogenesis.

    Staining:

    Article Title: Evaluation and experimental monitoring of calcium in plasma membrane inner nanoscale cytoplasm regions.
    Article Snippet: .. For some experiments, before staining, HEK cells were transiently transfected with an expression vector encoding NCX1 (Rat NCX1.1 (pcDNA3.1+), Addgene Cat. #75190) or with GCaMP6s (pGP-CMV-GCaMP6s-CAAX, Addgene Cat. #52228) using GenJect-40 reagent (dia-m.ru, Russia) according to standard manufacturer protocol. ..

    Article Title: Evaluation and experimental monitoring of calcium in plasma membrane inner nanoscale cytoplasm regions
    Article Snippet: .. For some experiments, before staining, HEK cells were transiently transfected with an expression vector encoding NCX1 (Rat NCX1.1 (pcDNA3.1+), Addgene Cat. #75190) or with GCaMP6s (pGP-CMV-GCaMP6s-CAAX, Addgene Cat. #52228) using GenJect-40 reagent (dia-m.ru, Russia) according to standard manufacturer protocol. ..

    Inhibition:

    Article Title: Insular input to the prelimbic cortex underlies social affective behavior in rats
    Article Snippet: .. Here we made deposits of the Gq coupled chemogenetic receptor hM3Dq, (AAV5-hSyn-hM3Dq-mCherry; Addgene Cat. No. 50474-AAV5), in either aIC or pIC with cannulas placed in the PL exactly as described for the terminal inhibition experiments described in . ..

    Sequencing:

    Article Title: Lentiviral-based vectors and related systems and methods for eukaryotic gene editing
    Article Snippet: 18 pSaCas93xms2 Plasmid expressing A synthetic dsDNA oligo was generated by annealing SaCas9 mRNA with oligo 3xloop-F (SEQ ID NO: 45) and oligo 3xloop-R three MS2 stem loops at (SEQ ID NO: 46), which was then inserted by Infusion TM the 3′ UTR reaction (Clontech)into the EcoRI site of pSaCas9- 2xMS2. .. 19 pSaCas912xms2 Plasmid expressing The EcoR1-Xho1 fragment from pSL-MS2-12X SaCas9 mRNA with (Addgene Cat. No. 27119), encoding the 12 MS2 stem twelve MS2 stem loops loops, was inserted between the EcoR1-SalI sites of at the 3′ UTR pSaCas9-1xMS2, which is after the stop codon of SaCas9 but before the polyA signal sequence. ..



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